电离辐射通过转化生长因子-β-介导的上皮-上皮转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简短 ：反思紫外光是否可通过转化内皮细胞-β（TGF-β）-特异性的上皮-间质转换 (EMT)来加强肿瘤细胞膜的侵入迁移。使用总和2Gy(60)Coγ线或紫外光源自有机体器官的6种肿瘤细胞膜，记录与EMT相关的变异，这都有分别来进行显微镜新技术，酶质索科利夫卡工具，免疫荧光新技术，划痕试验车和Transwell小室试验车来观察并扫描细胞膜组织形态，EMT标记，侵入迁移意志力等。采用酶联免疫吸附法扫描这些肿瘤细胞膜中TGF-β酶水平，来进行值得注意诱导剂SB431542来评估TGF-β频谱通路在紫外光EMT中的起着。经过总和为2Gy紫外光的肿瘤细胞膜中存在间叶细胞膜的表示，与；也紫外光组相比其上皮标记减少，间叶细胞膜标记上升，同时其侵入转到意志力增强，TGF-β酶水平也降低。大幅度发现由A549紫外光游离的EMT可通过对TGF-β频谱诱导引发逆转。这些结果表明TGF-β特异性的EMT在紫外光游离增强肿瘤细胞膜侵入转到意志力中起着关键性。

编者： lizexiu